lv cre | Delivery of the Cre recombinase by a self lv cre Cell type–specific gene inactivation in GluA2 + and GluA4 + neurons via the Cre recombinase system is an important application of GluA2/4-LV. Therefore, we packaged a transfer vector.
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0 · The Use of Lentiviral Vectors and Cre/loxP to Investigate the
1 · LV
2 · Delivery of the Cre recombinase by a self
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For manipulation of gene expression using floxed mice, a Cre-recombinase expressing viral vector (LV-Cre) can be created by replacing the GFP coding sequence with .Plasmid LV-Cre pLKO.1 from Dr. Elaine Fuchs's lab contains the insert Cre recombinase and is published in Nat Med. 2010 Jul . 16(7):821-7. This plasmid is available through Addgene. The Cre recombinase (Cre) from bacteriophage P1 is an important tool for genetic engineering in mammalian cells. We constructed lentiviral vectors that efficiently deliver Cre in . For manipulation of gene expression using floxed mice, a Cre-recombinase expressing viral vector (LV-Cre) can be created by replacing the GFP coding sequence with the coding sequence for Cre-recombinase (Heldt et al., 2007). In a typical experiment, mice are injected intra-cranially into specific brain areas with 1–2 μl of virus bilaterally .
The Use of Lentiviral Vectors and Cre/loxP to Investigate the
LV
Delivery of the Cre recombinase by a self
Plasmid LV-Cre pLKO.1 from Dr. Elaine Fuchs's lab contains the insert Cre recombinase and is published in Nat Med. 2010 Jul . 16(7):821-7. This plasmid is available through Addgene.
Cell type–specific gene inactivation in GluA2 + and GluA4 + neurons via the Cre recombinase system is an important application of GluA2/4-LV. Therefore, we packaged a transfer vector.LV-Cre efficiently transduced CV-1 cells (Fig. 1C). Figure 1. Lentiviral vectors and LV-Cre-mediated recombination in vitro and in vivo. (A) Schematic representation of the lentiviral vectors used in this study. (Top) The Cre lentiviral vector (LV-Cre) contains a Cre expression cassette with an nls. Expression of Cre is driven by the CMV promoter. The Cre recombinase (Cre) from bacteriophage P1 is an important tool for genetic engineering in mammalian cells. We constructed lentiviral vectors that efficiently deliver Cre in vitro and in vivo. Surprisingly, we found a significant reduction in proliferation and an accumulation in the G 2 /M phase of Cre-expressing cells.To minimize the toxic effect of Cre, we designed a self-deleting lentiviral vector (LV-Cre-SD), which contains a Cre expression cassette and a single loxP site incorporated into the U3 region of the 3’ long terminal repeat (LTR).
Kidney collecting duct (CD)-specific aquaporin-3 (AQP3)-knockdown mice were generated by consecutive injection of Hoxb7-Cre-expressing lentivirus (LV-Hoxb7 Cre) and loxP-AQP3 shRNA-expressing lentivirus (LV-loxP shAQP3) in adult C57BL6/J mice.Hand-crafted in small batches. Highest quality dairy and ingredients. Vegan-friendly options. Las Vegas and Beyond.
Cells infected with LV-CRE (moi = 70) expressed CRE recombinase within 24 h, with a peak of expression 48 h after infection; CRE recombinase levels decreased thereafter and were almost undetectable beyond 96 h after infection (data not shown).LV-Cre—that Selectively Infects Fos+ Neurons To efficiently and selectively infect neurons that express Fos/TVA, we generated a lenti-hSyn-Cre (synapsin promoter driving Cre recombinase expression) construct and packaged pseudotyped LVs with either the wild-type EnvA coat protein, or with several mutated forms of the EnvA (EnvAM4, EnvAM5 . For manipulation of gene expression using floxed mice, a Cre-recombinase expressing viral vector (LV-Cre) can be created by replacing the GFP coding sequence with the coding sequence for Cre-recombinase (Heldt et al., 2007). In a typical experiment, mice are injected intra-cranially into specific brain areas with 1–2 μl of virus bilaterally .Plasmid LV-Cre pLKO.1 from Dr. Elaine Fuchs's lab contains the insert Cre recombinase and is published in Nat Med. 2010 Jul . 16(7):821-7. This plasmid is available through Addgene.
Cell type–specific gene inactivation in GluA2 + and GluA4 + neurons via the Cre recombinase system is an important application of GluA2/4-LV. Therefore, we packaged a transfer vector.LV-Cre efficiently transduced CV-1 cells (Fig. 1C). Figure 1. Lentiviral vectors and LV-Cre-mediated recombination in vitro and in vivo. (A) Schematic representation of the lentiviral vectors used in this study. (Top) The Cre lentiviral vector (LV-Cre) contains a Cre expression cassette with an nls. Expression of Cre is driven by the CMV promoter. The Cre recombinase (Cre) from bacteriophage P1 is an important tool for genetic engineering in mammalian cells. We constructed lentiviral vectors that efficiently deliver Cre in vitro and in vivo. Surprisingly, we found a significant reduction in proliferation and an accumulation in the G 2 /M phase of Cre-expressing cells.To minimize the toxic effect of Cre, we designed a self-deleting lentiviral vector (LV-Cre-SD), which contains a Cre expression cassette and a single loxP site incorporated into the U3 region of the 3’ long terminal repeat (LTR).
Kidney collecting duct (CD)-specific aquaporin-3 (AQP3)-knockdown mice were generated by consecutive injection of Hoxb7-Cre-expressing lentivirus (LV-Hoxb7 Cre) and loxP-AQP3 shRNA-expressing lentivirus (LV-loxP shAQP3) in adult C57BL6/J mice.Hand-crafted in small batches. Highest quality dairy and ingredients. Vegan-friendly options. Las Vegas and Beyond.
Cells infected with LV-CRE (moi = 70) expressed CRE recombinase within 24 h, with a peak of expression 48 h after infection; CRE recombinase levels decreased thereafter and were almost undetectable beyond 96 h after infection (data not shown).
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lv cre|Delivery of the Cre recombinase by a self
